Search results for "CATABOLISM GENES"

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Fitness drift of an atrazine-degrading population under atrazine selection pressure.

2008

International audience; Pseudomonas sp. ADP harbouring the atrazine catabolic plasmid ADP1 was subcultured in liquid medium containing atrazine as sole source of nitrogen. After approximately 320 generations, a new population evolved which replaced the initial population. This newly evolved population grew faster and degraded atrazine more rapidly than the initial population. Plasmid profiles and Southern blot analyses revealed that the evolved strain, unlike the ancestral strain, presented a tandem duplication of the atzB gene encoding the second enzyme of the atrazine catabolic pathway responsible for the transformation of hydroxyatrazine to N-isopropylammelide. This duplication resulted …

DNA BacterialPopulationBiologyMicrobiologyPSEUDOMONAS SP03 medical and health scienceschemistry.chemical_compoundPlasmidGene DuplicationPseudomonasGene duplicationELEMENTSDirect repeatAtrazineInsertion sequenceSelection GeneticADAPTATIONeducationEcology Evolution Behavior and Systematics030304 developmental biologyGenetics0303 health scienceseducation.field_of_study030306 microbiologySALMONELLA-TYPHIMURIUMSTRAIN ADPCATABOLISM GENESTransformation (genetics)Blotting Southern[SDV.MP]Life Sciences [q-bio]/Microbiology and ParasitologychemistryGenes BacterialBACTERIADNA Transposable ElementsGROWTHAtrazineTandem exon duplicationPLASMIDRESISTANCEPlasmidsEnvironmental microbiology
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